GSK3 alpha (GSK3A) pTyr278/215 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6D3]

CAT#: AM20216BT-N

GSK3 alpha (GSK3A) pTyr278/215 (incl. pos. control) mouse monoclonal antibody, clone 6D3, Biotin

Specifications

Product Data

• Clone Name: 6D3
• Applications: WB
• Recommended Dilution: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
  Included Positive Control: Cell lysate from pervanadate treated HepG2 cells (See Protocols for more details).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Phosphopeptide conjugated to hemocyanin
  Epitope: Phosphotyrosine 215, Phosphotyrosine 278
• Specificity: This antibody specifically recognizes GSK3beta phosphorylated at Tyr215 at 46 kDa and GSK3alpha phosphorylated at Tyr278 at 54 kDa in Western blot applications.
• Formulation: PBS with 0.09% Sodium Azide/PEG and Sucrose.
  Label: Biotin
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.
• Conjugation: Biotin
• Storage: Aliquote and freeze in liquid nitrogen at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens glycogen synthase kinase 3 alpha (GSK3A)
• Database Link:
  Entrez Gene 606496 MouseEntrez Gene 2931 Human
• Background: Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling.
• Synonyms: Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A
• Note: Protocol: Positive Control Provided.
  Cell lysate from pervanadate-treated HepG2 cells
  
  Description: Cell lysate from pervanadate-treated HepG2 cells, hepatocellular carcinoma (Human)
  
  Format: Lyophilized cell lysate from from HepG2 cells.
  Serum starved cells were treated for 15min with pervanadate.
  
  Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway