Raptor (RPTOR) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10E10]

CAT#: AM20221PU-N

Raptor (RPTOR) (incl. pos. control) mouse monoclonal antibody, clone 10E10, Purified

Product Images

Detection of endogenous Raptor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20221PU-N Raptor antibody (Clone 10E10) at 0.5 ug/ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT-93 Lane 8: Neuro 2A Lane 9: 3T3

Specifications

Product Data

• Clone Name: 10E10
• Applications: WB
• Recommended Dilution: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
  Included Positive Control: Cell lysate from untreated HeLa cells (See Protocols for more details).
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide conjugated to hemocyanin derived from Raptor Sequence.
• Specificity: Recognizes Raptor.
• Formulation: 1ml PBS, containing 0.09% Sodium Azide/PEG and Sucrose.
  State: Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore with 1 ml H₂O (15 min, RT).
• Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.
• Gene Name: Homo sapiens regulatory associated protein of MTOR complex 1 (RPTOR), transcript variant 1
• Database Link:
  Entrez Gene 74370 MouseEntrez Gene 287871 RatEntrez Gene 57521 Human
• Background: The mTOR pathway (mammalian target of rapamycin) coordinates nutrient signals with growth factor dependent signaling. Recent data revealed that mTOR is organized in two independent protein complexes: The complex containing mTOR, GbL and RAPTOR is target of rapamycin while the complex containing mTOR, GbL and RICTOR is insensitive to rapamycin. The mTOR /RICTOR complex phosphorylates PKBalpha/akt1 at Serine 473
• Synonyms: RPTOR, KIAA1303, Regulatory-associated protein of mTOR, P150 target of rapamycin TOR-scaffold protein
• Note: Mol. weight: 145 kDa
  
  Protocol: Positive Control Provided: Cell lysate from untreated Hela cells
  
  Description: Cell lysate from untreated HeLa cells, epitheloid cervical carcinoma (Human)
  
  Format: Lyophilized cell lysate from serum starved HeLa cells.
  
  Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway