Caspase-7 (CASP7) Mouse Monoclonal Antibody [Clone ID: 7-1-11]

CAT#: AM20344PU-N

Caspase-7 (CASP7) mouse monoclonal antibody, clone 7-1-11, Purified

Product Images

Caspase-7 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Colon followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.

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  Caspase-7 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Placenta followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.

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  Caspase-7 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.

Specifications

Product Data

• Clone Name: 7-1-11
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot.
• Reactivities: Canine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant Human CASP7.
• Specificity: Detects an ~37 kD protein, corresponding to the apparent molecular mass of the Caspase-7 on SDS-PAGE Immunoblots, in samples from Human, Mouse, Rat, Monkey, Dog, Sheep, Rabbit, Pig and Hamster origins.
  This antibody recognizes the unprocessed pro-caspase-7 (p35), caspase-7 lacking the short amino- terminal pro domain (32 kD) and the fully processed Caspase-7 (p19) in apoptotic cell extracts.
• Formulation: 10 mM Sodium Phosphate, pH 7.2, 50 mM Sodium Chloride with 0.1 mM PMSF
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G Chromatography.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens caspase 7 (CASP7), transcript variant a
• Database Link:
  Entrez Gene 12369 MouseEntrez Gene 64026 RatEntrez Gene 697633 MonkeyEntrez Gene 840 Human
• Background: Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
• Synonyms: CASP-7, CASP7, MCH3, CMH-1

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis