XPF (ERCC4) Mouse Monoclonal Antibody

CAT#: AM20500PU-N

XPF (ERCC4) mouse monoclonal antibody, Purified

Product Images

Human Uterus (formalin-fixed, paraffin-embedded) stained with ERCC4 antibody AM20500PU-N at 10 ug/ml after heat-induced antigen retrieval.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1 - 2 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2
• Clonality: Monoclonal
• Immunogen: Recombinant Human XPF protein.
• Specificity: This antibody recognizes Human Excision Repair Cross-complementing Rodent Repair Deficiency, Complementation Group 4 (ERCC4). Other species not tested.
• Formulation: 10mM PBS, pH 7.4
  State: Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.2% BSA
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Protein G Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2072 Human
• Background: The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3' of the DNA lesion while the ERCC1 / XPF complex makes the 5' incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.
• Synonyms: ERCC-4, ERCC11, XPF