EMA (MUC1) Mouse Monoclonal Antibody [Clone ID: EMA-39]

CAT#: AM20621PU-N

EMA (MUC1) mouse monoclonal antibody, clone EMA-39, Purified

Specifications

Product Data

• Clone Name: EMA-39
• Applications: IF, IHC, IP
• Recommended Dilution: Immunohistochemistry on Paraffin-Embedded Sections: 2-4 μg/ml, Human, By Heat.
  Immunohistochemistry on Frozen Sections: 2-4 μg/ml, Human.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human milk fat globule membranes.
• Specificity: This antibody reacts to CD227. No cross reactivity with other proteins.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Add 1ml of PBS buffer will yield a concentration of 0.1 mg/ml.
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 2
• Database Link:
  Entrez Gene 4582 Human
• Background: MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly Oglycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
• Synonyms: MUC-1, PEMT, Episialin, EMA, H23AG, PUM, DF3, CA 15-3

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane