Estrogen Receptor 1 (ESR1) (B region) Mouse Monoclonal Antibody [Clone ID: ER-B10]

CAT#: AM21055PU-N

Estrogen Receptor 1 (ESR1) (B region) mouse monoclonal antibody, clone ER-B10, Ascites

Product Images

AM21055PU-N Estrogen receptor alpha antibody staining of Paraffin Human Hypothalamus Section (Clone ER-B10).

Specifications

Product Data

• Clone Name: ER-B10
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1/400.
  Immunohistochemistry on Paraffin Sections: 1/100. Micowave pretreatment for antigen retrieval is recommended.
  Suggested Positive Control: Human uterus or brain (hypothalamus) sections, Estrogen receptors are widely expressed in different tissue types.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: Peptide R151 to L165 from Human ER-alpha.
  The antigen is Estrogen Receptor alpha epitope is located in the B region.
• Specificity: This Monoclonal antibody ER-B10 recognizes an epitope in the B region of the Human Estrogen Receptor alpha.
  Does not react with Chicken Tissue.
  Antigen Distribution: The antigen has been described in endometrium, breast cancer cells, ovarian stroma cells, and the hypothalamus. In males, ER-alpha protein is found in the epithelium of the efferent ducts.
• Formulation: State: Ascites
  State: Liquid Undiluted Ascites
  Preservative: 0.01% Kathon as a preservative
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens estrogen receptor 1 (ESR1), transcript variant 1
• Database Link:
  Entrez Gene 2099 Human
• Background: The two different forms of the estrogen receptor, usually referred to as α and β, can form hormone-activated dimers, and, since the two forms are coexpressed in many cell types, the receptors may form ERα (αα) or ERβ (ββ) homodimers or ERαβ (αβ) heterodimers. Estrogen receptor alpha and beta show significant overall sequence homology, and both are composed of five domains (A-F domains listed from the N- to C-terminus).
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors