CD62E (SELE) Rat Monoclonal Antibody [Clone ID: 4D10]

CAT#: AM26048FC-N

CD62E (SELE) rat monoclonal antibody, clone 4D10, FITC

Specifications

Product Data

• Clone Name: 4D10
• Applications: FC
• Recommended Dilution: Has been reported to work in FACS.
  Suggested positive control: Human endothelial cells in acutely inflamed tonsil.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: LPS-stimulated human endothelial cells
• Specificity: This antibody detects CD62E / E-Selectin.
• Formulation: Phosphate buffered saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.09% sodium azide as a preservative
  Label: FITC
  State: Liquid Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Affinity chromatography
• Conjugation: FITC
• Storage: Store the antibody at 2-8 °C.
• Stability: Shelf life: six months from despatch.
• Gene Name: Homo sapiens selectin E (SELE)
• Database Link:
  Entrez Gene 6401 Human
• Background: CD62E belongs to the selectin family and is thought to mediate rolling of myeloid cells and a subset of memory T cells on activated endothelium.
  The antigen is a 589 amino acid transmembrane protein. It consists of a C-type lectin-like domain, an epidermal growth factor (EGF)-like domain, six short consensus repeat (SCR) domains, a transmembrane domain and a cytoplasmic tail. CD62E is a counter-receptor for sialyl Lewis X, sialyl Lewis a and CLA (cutaneous lymphocyte antigen). It is involved in adhesion between myeloid cells and activated endothelium, and supports leucocytes rolling. Some skin-associated memory T cells express CLA, and it may be that CD62E is also involved in their homing to cutaneous sites of chronic inflammation. In addition, CD62E may also be involved in tumour metastasis. A soluble form of CD62E has been reported to mediate angiogenesis in pathological disorders.
• Synonyms: SELE, ELAM1, LECAM2
• Note: Antigen distribution:
  Isolated cells: Positive on LPS, TNFα, IL-1, or TPA stimulated endothelial cells (HUVECs) in tissue culture. No cross reactivity with other cell types known.
  Tissue sections: Strongly positive on human endothelial cells in acutely inflamed tissues such as atopic dermatitis or contact dermatitis 24h after sensitization; in the healthy spleen and lymph node. Few single cells express the 4D10 antigen. The antigen is generally absent in chronically inflamed tissues.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)