Vcam1 Rat Monoclonal Antibody [Clone ID: 6C7.1]

CAT#: AM26309PU-N

Vcam1 rat monoclonal antibody, clone 6C7.1, Purified

Specifications

Product Data

• Clone Name: 6C7.1
• Applications: FC, FN, IF, IHC, IP
• Recommended Dilution: Immunohistochemistry on frozen sections.
  Flow cytometry: The typical starting working dilution is 1:50.
  Functional assays.
  Immunoflourescence: The typical starting working dilution is 1:50.
  Immunoprecipitation.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Mouse endothelial cells
• Specificity: The monoclonal antibody 6C7.1 recognizes mouse vascular cell adhesion molecule (VCAM-1).
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
• Concentration: 0.1 mg/ml
• Purification: Protein G
• Database Link:
  Entrez Gene 22329 Mouse
• Background: VCAM-1 (~ 81 kDa) is a member of a subclass of the immunoglobulin superfamily (IgSF). IgSF members are ligands for integrins. Cell adhesion molecules (CAMs) have important roles in the immune response, immune surveillance and cell-cell recognition, especially in leukocyte-endothelial cell adhesion. CAMs on the surface of leukocytes and endothelial cells are actively involved in the recruitment of specific leukocyte subsets into different tissues. VCAM-1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Cell adhesion molecules, like VCAM-1, are upregulated on cerebral vessels during inflammatory conditions of the central nervous system such as experimental autoimmune encephalomyelitis (EAE), a model system for multiple sclerosis. Administration of monoclonal antibody 6C7.1 has been shown to inhibit or diminish clinical or pathological signs of EAE. VCAM-1 is a receptor for encephalomyocarditis virus on murine vascular endothelial cells. Expression of VCAM-1 on vascular endothelial cells is induced by TNF-alpha, IL-1, IFN-gamma or endotoxin. VCAM-1 is a ligand for the integrins alpha4beta1 (VLA-4) and alpha4beta7 (LPAM-1). These integrins are constitutively expressed by thymocytes, lymphocytes and monocytes. VCAM-1/VLA-4 interaction may play a pathophysiological role in immune responses and as well as in leukocyte emigration to sites of inflammation.
• Synonyms: V-CAM 1, INCAM-100, L1CAM, VCAM-1