Amyloid beta (1-42 specific) Mouse Monoclonal Antibody [Clone ID: CA9 10C11]

CAT#: AM26394PU-L

Amyloid beta (1-42 specific) mouse monoclonal antibody, clone CA9 10C11, Aff - Purified

Product Images

Detection of Abeta 42 peptide using antibody Clone 10C11 at 2 g/ml dilution

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  Detection of Abeta 42 on 10 ng of peptide per lane using antibody Clone CA9 10C11 at 1 g/ml dilution.

Specifications

Product Data

• Clone Name: CA9 10C11
• Applications: ELISA, WB
• Recommended Dilution: ELISA: For sandwich ELISA use clone NT 4A2, Cat.-No AM26042PU-N as cupture antibody. The antibody can detect Aβ42 in Sandwich ELISA assay.
  Western blot
  Dot blot
• Reactivities: Human, Mouse, Primate, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: KLH conjugated to a short peptide corresponding to the C-terminal sequence of beta amyloid peptide 42
• Specificity: This antibody specifically recognizes the C-terminal sequence of beta amyloid peptide 42 and full length beta amyloid peptide 42.
  The antibody does not cross react with beta amyloid peptide 40 in dot blotting and ELISA. Cross-reactivity to beta amyloid peptide 43 is less than 1% in ELISA.
• Formulation: 0.01M PBS pH7.2
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with Double distillated water is recommended and to adjust the final concentration to 1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Amyloid beta peptide 42 (Aβ42) is best known for its role in the formation of senile plaques in the brain of patients with Alzheimer’s disease. Aβ42 and Aβ40 are the two major amyloid peptides that are produced after cleavage of amyloid precursor protein by secretases. Aβ42 (42 amino acids) is very fibrillogenic. The beta pleated structure of Aβ42 constituents the initial and key component of the insoluble amyloid fibril in senile plaque. It is widely accepted that Aβ42 contributes to the pathogenesis of Alzheimer’s disease. One proposition is that the deposition of amyloid fibril onto the brain tissue results in Alzheimer’s disease. Another is that the neurotoxicity of Aβ42 oligomer is the cause of the disease.