Retinoic Acid Receptor alpha (RARA) (1-463) Mouse Monoclonal Antibody [Clone ID: 2C9-1F8]

CAT#: AM31027PU-N

Retinoic Acid Receptor alpha (RARA) (1-463) mouse monoclonal antibody, clone 2C9-1F8, Purified

Product Images

RARA monoclonal antibody clone 2C9-1F8 Western Blot analysis of RARA expression in A-431.

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  Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to RARA on A-431 cell. [antibody concentration 10 ug/ml]

Specifications

Product Data

• Clone Name: 2C9-1F8
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: RARA (AAH08727, 1 a.a. ~ 463 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to full length RARA.
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Protein A chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens retinoic acid receptor alpha (RARA), transcript variant 1
• Database Link:
  Entrez Gene 5914 Human
• Background: Retinoic acid receptor alpha (RAR alpha), a NR1 Thyroid Hormone-Like Receptor, has a key effect on the development of acute promyelocytic leukemia (APL). APL results from chromosomal translocation of RAR alpha to various partner genes, including the promyelocytic leukemia (PML) gene on 15q22, the promyelocytic leukemia zinc finger (PLZF) gene on 11q23, the nucleophosmin (NPM) gene on 5q35, the nuclear mitotic apparatus (NuMA) gene on 11q13, and the signal transducer and activator of transcription STAT5b. The aberrant RAR alpha fusion proteins contribute to leukemic transformation by dominant inhibition of the expression of target genes that are important for cellular differentiation. Four alternatively spliced isoforms of RAR alpha have been documented in humans (RAR alpha1, RAR alpha2, RAR alpha1DeltaBC, and RAR alpha1DeltaB), one of which, RAR alpha1DeltaB, does not code for a functional receptor.
• Synonyms: NR1B1, Retinoic acid receptor alpha, Nuclear receptor subfamily 1 group B member 1

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer