FZR1 Mouse Monoclonal Antibody [Clone ID: 4C4]

CAT#: AM31770PU-N

FZR1 mouse monoclonal antibody, clone 4C4, Purified

Product Images

FZR1 monoclonal antibody, clone 4C4. Western Blot analysis of FZR1 expression in Hela NE.

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  Detection limit for recombinant GST tagged FZR1 is 1 ng/ml as a capture antibody.

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  Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Clone Name: 4C4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: FZR1 antibody was raised against fZR1 (AAH13413, 1 a.a. ~ 494 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Specificity: This antibody reacts to FZR1.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Affinity chromatography on Protein A
• Gene Name: Homo sapiens fizzy/cell division cycle 20 related 1 (FZR1), transcript variant 3
• Database Link:
  Entrez Gene 51343 Human
• Background: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
• Synonyms: Cdh1/Hct1 homolog, CDC20-like protein 1, Fizzy-related protein homolog, FYR, FZR, KIAA1242

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis