CD1 (CD1A) Rabbit Monoclonal Antibody [Clone ID: EP3622]

CAT#: AM32240PU-N

CD1 (CD1A) rabbit monoclonal antibody, clone EP3622, Purified

Specifications

Product Data

• Clone Name: EP3622
• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on ParaffinSections.
  Recommended dilution: 1/25-1/100.
  Positive Control: Thymus.
  Staining pattern: Membranous.
  Preparation and Pretreatment
  1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
  2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) technique in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
  3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG1
• Clonality: Monoclonal
• Formulation: PBS, pH 7.4
  State: Purified
  State: Liquid purfied Tissue Culture Supernatant
  Stabilizer: 0.9% BSA
  Preservative: 0.09% Sodium Azide
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD1a molecule
• Database Link:
  Entrez Gene 909 Human
• Background: CD1a, a protein of 43 to 49 kD, has been shown to be expressed on dendritic cells and cortical thymocytes. Langerhans cells in the skin and some epithelia also express this protein. This antigen is expressed on cells comprising Langerhans cell histiocytosis and Langerhans cell sarcoma. Anti-CD1a has been used to differentiate various cutaneous lymphomas (T-cell) from B-cell lymphomas and pseudolymphomas.
• Synonyms: T6/Leu-6, hTa1