CD36 Mouse Monoclonal Antibody [Clone ID: VM58]

CAT#: AM32332PU-N

CD36 mouse monoclonal antibody, clone VM58, Purified

Specifications

Product Data

• Clone Name: VM58
• Applications: FN, IF, IHC
• Recommended Dilution: Functional studies on CD36.
  The antibody can be used in:
  - Indirect Immunofluorescence staining with analysis by Cytofluorometry or Fluorescence microscopy.
  - Immunohistology.
  For fluorescence technique on cells, fixation with 1% paraformaldehyde for 5 min. is advised.
  The antibody cannot be used in Western blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human monocytes.
• Specificity: This antibody reacts with platelets, monocytes, macrophages, erythroblasts and (weakly) with B-cells.
  In immunohistology the monoclonal antibody reacts with some endothelial cells, adipocytes and the granular layer of the skin.
  Clone VM58 induces Fc-receptor-dependent platelet aggregation after binding to platelet.
  The heterogeneity of the response found in healthy individuals is dependent on the polymorphism of the Fc RII on platelets.
• Formulation: PBS
  State: Purified
  State: Lyophilized purified IgG fraction
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore with 1 ml sterile water to receive an IgG concentration of ~0.2 mg/ml (sufficient for 100 tests)
• Concentration: lot specific
• Purification: Ammonium sulfate + DEAE-cellulose, gradient elution
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD36 molecule
• Database Link:
  Entrez Gene 948 Human
• Background: CD36, the GP IV (GP IIIb) molecule, has been shown to function as thrombospondin receptor on platelets and monocytes and as receptor for Plasmodium falciparum infected erythrocytes.
• Synonyms: Glycoprotein IIIb, PAS IV, PAS-4, Thrombospondin receptor, GP3B, GP4