HLA Class II DR Mouse Monoclonal Antibody [Clone ID: B-C10]

CAT#: AM32426FC-N

HLA Class II DR mouse monoclonal antibody, clone B-C10, FITC, Purified

Product Images

A typical staining pattern of lymphocytes with the B-C10 monoclonal antibody.

Specifications

Product Data

• Clone Name: B-C10
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl to label 10⁶ cells or 100 µl of whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: RPMI 8866 and DAUDI cell lines.
  Myeloma X63/AG.8653 x Balb/c spleen cells.
• Specificity: This Monoclonal antibody recognises the HLA Class II (DR) membrane.
• Formulation: PBS
  Label: FITC
  State: Purified
  State: Liquid purified IgG fraction
  Stabilizer: 5% BSA
  Preservative: 0.09% Sodium Azide
• Purification: Ion Exchange Chromatography
• Conjugation: FITC
• Background: Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC II alpha/beta dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (beta-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles.
• Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR