ICT1 (MRPL58) (30-206) Mouse Monoclonal Antibody [Clone ID: AT1E9]

CAT#: AM50048PU-N

ICT1 (MRPL58) (30-206) mouse monoclonal antibody, clone AT1E9, Purified

Product Images

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ICT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT1E9
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA.
  Western blot: Recommended starting dilution is 1:1000.
  Flow cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human ICT1 (30-206aa) purified from E. coli
• Specificity: This antibody detects ICT1 at aa30-206.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens mitochondrial ribosomal protein L58 (MRPL58), transcript variant 1
• Database Link:
  Entrez Gene 3396 Human
• Background: Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. This neoplastic-induced deviation is marked by a change in expression of several mRNAs. ICT1 is a member of the prokaryotic/mitochondrial release factor family whose expression is downregulated over 4 fold upon colon stem cell differentiation. This downregulation of ICT1 could lead to its use as a marker for detection of colon carcinomas.
• Synonyms: Digestion substraction 1, DS-1