PARP2 Mouse Monoclonal Antibody [Clone ID: AT29G4]

CAT#: AM50055PU-S

PARP2 mouse monoclonal antibody, clone AT29G4, Purified

Product Images

The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT29G4
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA.
  Flow Cytometry
  Immunocytochemistry/Immunofluorescence.
  Western blot / Immunoblot.
  The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:500.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human PARP2 (233-583aa) purified from E. coli
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-A affinity chromatography
• Gene Name: Homo sapiens poly(ADP-ribose) polymerase 2 (PARP2), transcript variant 1
• Database Link:
  Entrez Gene 10038 Human
• Background: PARP2 is poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.
• Synonyms: PARP-2, ADPRT2, ADPRTL2, Poly [ADP-ribose] polymerase 2, PARP 2, EC=2.4.2.30, pADPRT-2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair