TGF alpha (TGFA) Mouse Monoclonal Antibody [Clone ID: SPM542]

CAT#: AM50174PU-T

TGF alpha (TGFA) mouse monoclonal antibody, clone SPM542, Purified

Product Images

Formalin-paraffin human Testicular Carcinoma Stained with TGF alpha MAb (SPM542)

Specifications

Product Data

• Clone Name: SPM542
• Applications: FC, IF, IHC
• Recommended Dilution: Western Blotting (Not tested).
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunohistochemistry on Formalin-Fixed Paraffin Sections: 1-2 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast cancer, Melanoma.
• Reactivities: Human, Rabbit, Zebrafish
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: A 10-amino acid synthetic peptide (aa34-43) from Human TGFα.
• Specificity: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin.
  The staining with this Monoclonal Antibody is completely blocked by the peptide used for raising this antibody. TGFα (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
  Cellular Localization: Cytoplasmic and Extracellular (secreted).
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purification: Protein A/G Chromatography
• Predicted Protein Size: ~6 kDa
• Database Link:
  Entrez Gene 7039 Human
• Synonyms: Transforming growth factor alpha, ETGF