HINT2 Mouse Monoclonal Antibody [Clone ID: AT5B10]

CAT#: AM50638PU-N

HINT2 mouse monoclonal antibody, clone AT5B10, Purified

Product Images

The recombinant human HINT2 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HINT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HINT2 Recombinant protein Lane 2. : HepG2 cell lysate Lane 3. : U87MG cell lysate Lane 4. : Mouse brain lysate

Specifications

Product Data

• Clone Name: AT5B10
• Applications: ELISA, FC, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human HINT2 (18-163aa) purified from E. coli.
• Formulation: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Protein-A affinity chromatography
• Gene Name: Homo sapiens histidine triad nucleotide binding protein 2 (HINT2)
• Database Link:
  Entrez Gene 84681 Human
• Background: Histidine triad nucleotide binding protein 2 (HINT2) is a mitochondrial protein. This protein is an AMP-lysine hydrolase and phosphoamidase and may contribute to tumor suppression. This protein is a member of the HIT superfamily and Hint subfamily, which are characterized as nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides. HINT2 regulates steroidogenesis through calcium-dependent and calcium-independent siglling pathways that may serve to maintain a favorable mitochondrial potential. Its role in calcium homeostasis may also contribute to its proapoptotic function in hepatocytes and other non-steroidogenic cells, though the exact mechanism remains unclear.
• Synonyms: HINT-2, HINT-3, HIT-17kDa, PKCI-1-related HIT protein