SIGLEC5 / SIGLEC14 Mouse Monoclonal Antibody [Clone ID: 1A5]

CAT#: AM60041FC-N

SIGLEC5 / SIGLEC14 mouse monoclonal antibody, clone 1A5, FITC, Purified

Product Images

Human peripheral blood monocytes stained with SIGLEC5 / SIGLEC14 antibody Cat.-No.AM60041FC-N.

Specifications

Product Data

• Clone Name: 1A5
• Applications: FC
• Recommended Dilution: Flow cytometry: Neat-1/10, use 10µl of this working dilution to label 10e6 cells in 100µl.
• Reactivities: Chimpanzee, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: SIGLEC5-Fc protein, consisting of the full-length extracellular region of human SIGLEC5, fused with the Fc region of human IgG1
• Specificity: This antibody recognises human SIGLEC5 / CD170. Clone 1A5 antibody is one of several SIGLEC5 antibodies which also recognises human SIGLEC14 (Angata, T. et al., 2006).
• Formulation: PBS containing 0.09% Sodium azide and 1% BSA
  Label: FITC
  State: Purified
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: 0.1 mg/ml
• Purification: Affinity chromatography on Protein G
• Conjugation: FITC
• Background: Siglec-5 shares an almost identical sequence with Siglec-14 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species.
  Siglec-5, also known as CD170, is a sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), Siglec-5 mediates sialic-acid dependent binding to cells, and is as well acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases.
  Siglec-14 is a putative sialic-acid binding adhesion molecule and predominantly expressed in hematopoietic tissues, which have been shown to associate with the activating adapter protein DAP12.
• Synonyms: Q08ET2, O15389, CD170