Lactoferrin (LTF) Sheep Polyclonal Antibody

CAT#: AP01083HR-N

Lactoferrin (LTF) sheep polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: ELISA: 1/50.
  Immunohistochemistry on frozen sections.
  Immunohistochemistry on paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
  Recommended Positive Control: Mammary gland.
• Reactivities: Human
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Lactoferrin purified from milk.
• Specificity: This antibody recognises lactoferrin (LTF).
• Formulation: PBS, pH 7.4 containing 0.01% Thiomersal
  Label: HRP
  State: Liquid purified IgG
  Label: Horseradish Peroxidase
• Concentration: lot specific
• Purification: Protein G chromatography
• Conjugation: HRP
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: lactotransferrin
• Database Link:
  Entrez Gene 4057 Human
• Background: Lactoferrin is an 80kDa non-haem, iron-binding globular glycoprotein and member of the transferrin family present in milk and mucosal secretions, and also released from neutrophil granules during inflammation. LTF acts as a major first line defense against microbial infections, partly due to its ability to efficiently bind to and hence remove Fe3+ from the environment, but studies have also shown that LTF possesses bactericidal properties and proteolytic activity, capable of cleaving arginine-rich sequences within microbial proteins. Three isoforms of LTF have been identified: LTF-alpha, LTF-beta and LTF-gamma and receptors for LTF are expressed in the gastrointestinal tract, by macrophages, lymphocytes, neutrophils and platelets and also by some bacteria.
• Synonyms: LTF, LF, Lactoferrin, EC=3.4.21, Talalactoferrin