VEGFA Goat Polyclonal Antibody
Specifications
Product Data | |
Applications | ELISA, FN, WB |
Recommended Dilution | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10 ng/ml), a concentration of 0.05-0.1 µg/ml of this antibody is required. Sandwich ELISA: To detect Human VEGF by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml is required. In conjunction with Biotinylated Anti-Human VEGF (AP01112BT-S or AP01112BT-N) as a Detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human VEGF. Western Blot: To detect Human VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human VEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 5.0-15.0 µg/ml with 30 minutes at RT or an overnight incubation at 4°C. This antibody stained Formalin-Fixed, Paraffin-Embedded sections of Human breast invasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-Biotin labeling detection system. Heat induced antigen retrieval with pH6.0 Sodium Citrate buffer is recommended. |
Reactivities | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Highly pure (> 98%) E.coli derived recombinant Human VEGF165 (Cat.-No PA136) |
Specificity | This antibody recognizes Human VEGF. Other species not tested. |
Formulation | PBS, pH 7.2 State: Purified State: Sterile filtered, lyophilized Ig fraction |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Immunoaffinity Chromatography |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Synonyms | VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor |
Note | Centrifuge vial prior to opening. |
Reference Data |
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