MCP4 (CCL13) Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, FN, WB |
Recommended Dilution | ELISA: Indirect: To detect hMCP-4 by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4. Sandwich: To detect hMCP-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml is required. This In conjunction with Biotinylated Anti-Human MCP-4 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-4. Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-4 (100 ng/ml), a concentration of 8 - 12 µg/ml of this antibody is required. |
Reactivities | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Highly pure (> 98 %) recombinant human MCP-4 |
Specificity | This antibody detects Macrophage Chemotactic Protein-4. |
Formulation | PBS, pH 7.2 State: Aff - Purified State: Sterile filtered lyophilized Ig fraction |
Reconstitution Method | Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/ml. |
Purification | Immunoaffinity chromatography |
Storage | Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Database Link | |
Background | Human macrophage/monocyte chemotactic protein 4 (MCP4) is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP3, MCP4 elicits chemo-attraction of monocytes and T lymphocytes. |
Synonyms | C-C motif chemokine 13, CCL13, MCP4, NCC1, SCYA13, CK-beta-10, NCC-1, Monocyte chemotactic protein 4 |
Reference Data |
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