Apolipoprotein E (APOE) (Isoform E3) Rabbit Polyclonal Antibody

CAT#: AP01142BT-N

Apolipoprotein E (APOE) (Isoform E3) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human ApoE3 by Direct ELISA (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. This Biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3.
  Sandwich ELISA: To detect Human ApoE3 by Sandwich ELISA (using 100 µl/well) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotinylated antibody, in conjunction with Affinity purified anti-Human ApoE3 (AP01142PU-N or AP01142PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3.
  Western blot: To detect Human ApoE3 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: E.Coli derived recombinant Human ApoE3.
• Specificity: This antibody detects ApoE3. Cross reacts with Human ApoE2 and Human ApoE4 in both Sandwich ELISA and Western Blot.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction.
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Storage: Store the lyophilized antibody at -20°C.
  Following reconstitution it is stable for two weeks at 2-8°C.
  Frozen aliquots are stable for 6 months when stored at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: 6 month from despatch.
• Database Link:
  Entrez Gene 348 Human
• Background: Apolipoprotein E3 mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo E receptor (chylomicron remnant) of hepatic tissues.
• Synonyms: ApoE, Apo-E
• Note: Centrifuge vial prior to opening.
  Molecular Weight: ~150 kDa