Melanoma Inhibitory Activity (MIA) Rabbit Polyclonal Antibody

CAT#: AP01143BT-N

Melanoma Inhibitory Activity (MIA) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human MIA (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml of this antibody is required. It allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA.
  Sandwich ELISA: To detect Human MIA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human MIA (AP01143PU-S or AP01143PU-N) as a Capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIA.
  Western blot: To detect Human MIA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) recombinant Human MIA
• Specificity: This antibody detects MIA.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Database Link:
  Entrez Gene 8190 Human
• Background: Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.
• Synonyms: Melanoma inhibitory activity