Artemin (ARTN) Rabbit Polyclonal Antibody

CAT#: AP01145BT-S

Artemin (ARTN) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Indirect ELISA: To detect Human Artemin (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human Artemin.
  Sandwich ELISA: To detect Human Artemin (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human Artemin (Cat.N AP0145PU) as a Capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human Artemin.
  Western blot: To detect Human Artemin this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Artemin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98%) derived recombinant Human Artemin
• Specificity: This antibody detects Artemin.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening.
  Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Database Link:
  Entrez Gene 9048 Human
• Background: Artemin is a member of the glial cell line-derived neurotophic factor (GDNF) family of ligands, a group of ligands within the TGF-beta superfamily of signalling molecules. GDNFs are unique in having neurotrophic properties and have potential use for gene therapy in neurodegenrative disease. Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. This protein is a ligand for the RET receptor and uses GFR-alpha 3 as a coreceptor. Four alternatively spliced transcripts have been described, two of which encode the same protein.
• Synonyms: Enovin, Neublastin, ARTN, EVN