beta Defensin 1 (DEFB1) Rabbit Polyclonal Antibody

CAT#: AP01151BT-S

beta Defensin 1 (DEFB1) rabbit polyclonal antibody, Biotin

Product Images

Western Blot (Unreduced) using BD1 Antibody Cat.-No AP01151B

• 

  Western Blot (Reduced) using BD1 Antibody Cat.-No AP01151B

• 

  Direct ELISA using BD1 Antibody Cat.-No AP01151B

• 

  Sandwich ELISA using BD1 Antibody Cat.-No AP01151B

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human BD-1 (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-1.
  Sandwich ELISA: To detect Human BD-1 (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human BD-1 (Cat.-No AP01151PU-N or AP01151PU-S) as a capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-1.
  Western blot: To detect Human BD-1 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BD-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: E. Coli derived recombinant Human beta Defensin-1
• Specificity: This antibody detects Human BD-1.
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  Entrez Gene 1672 Human
• Background: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis.
  The mature form of Beta defensin 1 is 36 amino acids.
• Synonyms: Beta-defensin 1, BD1, BD-1, HBD1, hBD-1, DEFB1, DEFB101