MIA2 Rabbit Polyclonal Antibody

CAT#: AP01153BT-S

MIA2 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: Direct: To detect hMIA-2 (using 100 μl/well antibody solution) a concentration of
  0.25 - 1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2.
  Sandwich: To detect hMIA-2 (using 100 μl/well antibody solution) a concentration of
  0.25 - 1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human MIA-2 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2.
  Western blot: To detect hMIA-2 this antibody can be used at a concentration of
  0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98 %) recombinant human MIA-2
• Specificity: This antibody detects MIA-2.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 -1 .0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Background: MIA2 (Melanoma inhibitory activity 2) is a secreted cytokine and a member of the MIA/OTOR family. It is predominantly expressed in hepatocytes and may play a role in the pathophysiology of liver disease. Elevated levels of MIA2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity. MIA2 may act as a tumor suppressor in hepatocellular carcinoma.
• Synonyms: Melanoma inhibitory activity protein 2