Histone H2A.X (H2AFX) Rabbit Polyclonal Antibody

CAT#: AP01346PU-N

Histone H2A.X (H2AFX) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Histone H2A.X antibody (Cat.-No.: AP01346PU-N) in extracts from HT29 cells.

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  Immunohistochemistry (IHC) analyzes of Histone H2A.X antibody (Cat.-No.: AP01346PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Histone H2A.X antibody detects endogenous levels of Histone H2A.X protein. (region surrounding Ser130)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 19 kDa
• Gene Name: Homo sapiens H2A histone family member X (H2AFX)
• Database Link:
  Entrez Gene 15270 MouseEntrez Gene 500987 RatEntrez Gene 3014 Human
• Background: H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.
• Synonyms: H2a/x, H2AFX, H2AX

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus