Endothelin 1 (EDN1) Sheep Polyclonal Antibody

CAT#: AP02048PU-S

Endothelin 1 (EDN1) sheep polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: ELISA (1/10000).
  Immunohistochemistry on Crysections.
  Immunohistochemistry on Paraffin Sections (1/10).
• Reactivities: Human
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Human Big Endothelin, KLH conjugated
• Specificity: This antobody detects Big Endothelin (aa 1-38) (100%), Big Endothelin Fragment (aa 22-38) (< 98%), Endothelin 1 (< 1%), Endothelin 2 (< 1%), Endothelin 3 (< 1%), Sarafotoxin (< 1%), ANP (< 1%).
• Formulation: 0.05 M Borate buffer pH 8,5
  State: Purified
  State: Lyophilized purified IgG fraction from Serum
• Reconstitution Method: Restore in 100 µl aqua bidest.
• Purification: Affinity Chromatography
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1906 Human
• Background: Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
• Synonyms: PPET1, EDN1, ET1, HDLCQ7, EDN1