p38 (MAPK14) pTyr182 Rabbit Polyclonal Antibody

CAT#: AP02505PU-N

p38 (MAPK14) pTyr182 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK antibody (Phospho-Tyr182).

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  Western blot analysis of extracts from NIH-3T3 (Line 1, 4, 7 and 10) and cos7 (Line 2, 5, 8 and 11 and K562 (Line 3, 6, 9 and 12) cells, untreated or treated with UV (20min), using P38 MAPK antibody (Lane 1, 2, 3, 4, 5 and 6) and P38 MAPK (pTyr182) antibody (Lane 7, 8, 9, 10, 11 and12).

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using P38 MAPK antibody.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500~1/1000.
  Immunohistochemistry: 1/50~1/100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human p38 MAPK around the phosphorylation site of tyrosine 182 (T-G-YP-V-A).
• Specificity: This antibody detects endogenous levels of P38MAPK only when phosphorylated at Tyrosine 182.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide.
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens mitogen-activated protein kinase 14 (MAPK14), transcript variant 1
• Database Link:
  Entrez Gene 1432 Human
• Synonyms: Mitogen-activated protein kinase 14, p38 alpha, MXI2, SAPK2A, CSBP, CSBP1, CSBP2, CSPB1
• Note: For western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, GnRH signaling pathway, Leukocyte transendothelial migration, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway, VEGF signaling pathway