Bax (beta isoform) Sheep Polyclonal Antibody

CAT#: AP05118PU-N

Bax (beta isoform) sheep polyclonal antibody, Purified

Product Images

Immunoprecipitation of in vitro translated Bax-alpha (1) and Bax-beta (2) using Bax antibody, cln 6A7, Bax-alpha native (3) and denatured protein (4) using Bax-beta antibody, and Bax-beta native (5) and denatured (6) protein using Bax-beta antibody.

Specifications

Product Data

• Applications: IP
• Recommended Dilution: Immunoprecipitation.
  Will NOT detect denatured protein by Western blot.
• Reactivities: Mouse
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 172 to 199 of the Bax beta protein
• Specificity: This antibody reacts to Bax-beta.
• Formulation: Phosphate buffered saline with 0.08% sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: Lot specific
• Database Link:
  Entrez Gene 12028 Mouse
• Background: Bax-beta is encoded by a 1.5 kb RNA species created by the retention of intron 5 in the bax mRNA transcript. The exon4/intron5 splice junction is evolutionarily conserved and is found in both the human and mouse bax coding regions. The 630 bp intron 5 contributes 60 amino acids to the Bax-beta protein before a stop codon is encountered during translation. The Bax-beta protein is 24 kDa in size and although it contains the BH1, BH2, and BH3 domains identical to Bax, it has a unique carboxyl terminus and does not have a hydrophobic transmembrane domain (Oltvai et al., 1993). Bax-beta mRNA has been found in many mouse tissues using RT-PCR. It is found co-expressed with Bax-alpha in lung, stomach, kidney, thymus, lymph node, bone marrow, pancreas, and spleen. Bax-beta mRNA was very abundant in brain and duodenum with almost no Bax-alpha mRNA detected in these tissues (Oltvai et al., 1993).
• Synonyms: Apoptosis regulator BAX, BCL2L4, Bcl2-L-4