TLR9 Rabbit Polyclonal Antibody

CAT#: AP05241PU-N

TLR9 rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 0.5-2 μg/ml.
  Positive Control: Jurkat Cell Lysate. Located in the membrane, and highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver (6).
  Immunohistochemistry on Paraffin Sections.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide corresponding to 15 amino acids near the center of human TLR9
• Specificity: This antibody detects TLR9.
• Formulation: Phosphate buffered saline with 0.02% Sodium Azide
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Antigen Affinity Chromatography
• Predicted Protein Size: 116 kDa
• Database Link:
  Entrez Gene 54106 Human
• Background: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences (3) and are localized in intracellular acidic compartments such as the phagolysosome (4). Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection (5), TLR9 is strictly dependent on MyD88 (3).
• Synonyms: Toll-like receptor 9, UNQ5798/PRO19605