E. coli J5 LPS Sheep Polyclonal Antibody

CAT#: AP05457PU-N

E. coli J5 LPS sheep polyclonal antibody, Serum

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: ELISA.
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Heat inactivated Escherichia coli J5 (Rc mutant; ATCC).
• Specificity: This antibody recognises the J5 mutant of E. coli 0:111 which lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).
• Formulation: State: Serum
  State: Liquid Serum
  Preservative: 0.09% Sodium Azide
• Background:

  Some form of LPS is a major component of the cell membrane of Gram negative bacteria, contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response from normal animal immune systems. LPS function has been under experimental research for several years due to its role in activating many transcriptional factors, which become active after stimulation with LPS. LPS also produces many types of mediators involved in septic shock.
  The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).

• Synonyms: Escherichia coli, EHEC, EPEC, ETEC