Cullin 2 (CUL2) Rabbit Polyclonal Antibody

CAT#: AP06078PU-N

Cullin 2 (CUL2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CUL-2 antibody (Cat.-No.: AP06078PU-N) at 1/500 dilution Lane 1:MCF-7 cell lysate Lane 2:Mouse liver tissue lysate Lane 3:Rat kidney tissue lysate

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  Immunohistochemical (IHC) analysis in paraffin-embedded human lung carcinoma tissue using Cullin-2 antibody (Cat-No. AP06078PU-N).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Cullin-2 protein (region surrounding Ile726).
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen (> 95% pure by SDS-PAGE)
• Predicted Protein Size: ~ 82, 84 kDa
• Gene Name: Homo sapiens cullin 2 (CUL2), transcript variant 3
• Database Link:
  Entrez Gene 71745 MouseEntrez Gene 8453 Human
• Background: Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of Cdc53 from yeast, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF(Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1 mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3 facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin- like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, or vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.
• Synonyms: CUL-2, CUL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis