EP300 Rabbit Polyclonal Antibody

CAT#: AP06548PU-N

EP300 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of p300 antibody in extracts from Hela cells.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human p300.
• Specificity: This antibody detects endogenous levels of p300 protein.
  (region surrounding Glu83)
• Formulation: PBS, pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE).
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~265.0 kDa
• Gene Name: Homo sapiens E1A binding protein p300 (EP300)
• Database Link:
  Entrez Gene 328572 MouseEntrez Gene 170915 RatEntrez Gene 2033 Human
• Background: Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A, bind to this element. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265 kDa nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.
• Synonyms: p300 HAT, KAT3B, Histone acetyltransferase p300

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Huntington's disease, Jak-STAT signaling pathway, Long-term potentiation, Melanogenesis, Notch signaling pathway, Pathways in cancer, Prostate cancer, Renal cell carcinoma, TGF-beta signaling pathway, Wnt signaling pathway