EP300 Rabbit Polyclonal Antibody

CAT#: AP06548PU-N

EP300 rabbit polyclonal antibody, Aff - Purified


USD 370.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Applications IHC, WB
Recommended Dilution Western blot: 1/500-1/1000.
Immunohistochemistry on paraffin sections: 1/50-1/200.
Reactivities Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide, corresponding to amino acids 50-100 of Human p300.
Specificity This antibody detects endogenous levels of p300 protein.
(region surrounding Glu83)
Formulation PBS, pH~7.2
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% by SDS-PAGE).
Preservative: 15 mM Sodium Azide
Concentration 1.0 mg/ml
Purification Affinity Chromatography using epitope-specific immunogen.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Predicted Protein Size ~265.0 kDa
Gene Name Homo sapiens E1A binding protein p300 (EP300)
Background Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A, bind to this element. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265 kDa nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.
Synonyms p300 HAT, KAT3B, Histone acetyltransferase p300
Reference Data
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Adherens junction, Cell cycle, Huntington's disease, Jak-STAT signaling pathway, Long-term potentiation, Melanogenesis, Notch signaling pathway, Pathways in cancer, Prostate cancer, Renal cell carcinoma, TGF-beta signaling pathway, Wnt signaling pathway

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.