Thymidylate Synthase (TYMS) Sheep Polyclonal Antibody

CAT#: AP08213PU-N

Thymidylate Synthase (TYMS) sheep polyclonal antibody, Purified

Product Images

Western Blot using Thymidylate Synthase Antibody: Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2000, 1:5000 and 1:10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-anti-Sheep IgG diluted 1:1000 and color development using TMB (TMBM-100) substrate for approximately 4.

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  Formalin-Fixed Paraffin-Embedded Human Thymus stained with using Thymidylate Synthase Antibod

Specifications

Product Data

• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: ELISA.
  Immunofluorescence.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Immunoprecipitation.
  Western Blot.
• Reactivities: Human
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant human Thymidylate Synthase (36 kD) produced in E. coli
• Specificity: This antibody recognizes Human Thymidylate Synthetase (TYMS).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 7298 Human
• Background: Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.
• Synonyms: TYMS, TSase, OK/SW-cl.29