DNMT1 (177-550) Chicken Polyclonal Antibody

CAT#: AP09035AF-N

DNMT1 (177-550) chicken polyclonal antibody, Azide Free

Product Images

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)

• 

  Placenta: Formalin-Fixed Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 1/2000.
• Reactivities: Human
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Recombinant human DNMT1 (aa 177-550)
• Specificity: This antibody reacts to DNA (cytosine-5-)-methyltransferase 1 (DNMT1) at aa 177-550.
• Formulation: PBS, without preservatives
  State: Azide Free
  State: Liquid purified ig
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1786 Human
• Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt 1, Dnmt 2 and Dnmt 3. Dnmt 1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt 2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt 3a and Dnmt 3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F 1, and HDAC 1. Dnmt 1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence specific DNA binding activity, as well as a growth regulatory pathway that is disrupted in nearly all cancer cells.
• Synonyms: MCMT, HsaI, AIM, CXXC9