Alkaline phosphatase / ALPI / IAP Goat Polyclonal Antibody

CAT#: AP09067PU-S

Alkaline phosphatase / ALPI / IAP goat polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA: 1/4000-1/20000.
  Western Blot: 1/500-1/2000.
  Immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
• Reactivities: Bovine
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Alkaline phosphatase from calf intestine
• Specificity: This antibody detects bovine alkaline phosphatase. Cross reactivity against alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-Goat Serum as well as purified and partially purified alkaline phosphatase [calf intestine].
• Formulation: 0.01M Sodium phosphate, 0.14M Sodium chloride, pH 7.4
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Background: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
• Synonyms: Intestinal alkaline phosphatase
• Note: Conjugates available. Please ask for details.