Alkaline phosphatase / ALPI / IAP Sheep Polyclonal Antibody

CAT#: AP09101AF-N

Alkaline phosphatase / ALPI / IAP sheep polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western Blot.
  ELISA: 1/1,000 to 1/5,000.
  Immunoprecipitation.
• Reactivities: Bovine
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Alkaline Phosphatase [Calf intestine]
• Specificity: This antibody reacts to Alkaline Phosphatase. No reaction was observed against the bacterial form of the enzyme.
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4
  State: Azide Free
  State: Lyophilized
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: 33.0 mg/ml (by Refractometry)
• Background: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
• Synonyms: Intestinal alkaline phosphatase