Alkaline phosphatase / ALPI / IAP Sheep Polyclonal Antibody

CAT#: AP09101PU-S

Alkaline phosphatase / ALPI / IAP sheep polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/2000.
  ELISA: 1/4000 to 1/20000.
  This antibody has been assayed against 1.0 ug of alkaline phosphatase [calf intestine] in a standard ELISA using peroxidase conjugated affinity purified anti-sheep IgG [H&L] (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested.
• Reactivities: Bovine
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Alkaline phosphatase from calf intestine
• Specificity: This antibody reacts to bovine alkaline phosphatase. Cross reactivity against alkaline phosphatase from other tissues and species may occur but have not been specifically determined.
  Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified alkaline phosphatase [calf intestine].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Background: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
• Synonyms: Intestinal alkaline phosphatase