Glucose Oxidase Sheep Polyclonal Antibody

CAT#: AP09102HR-S

Glucose Oxidase sheep polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/10,000-1/40,000.
  Western blot: 1/1,000-1/5,000.
  This antibody has been assayed against 1.0 µg of Glucose Oxidase [Aspergillus niger] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/3,000 of the reconstitution concentration is suggested.
• Host: Sheep
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Glucose Oxidase from Aspergillus niger
• Specificity: This antibody reacts to Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified Glucose Oxidase.
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized IgG fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Background: Glucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid.
• Synonyms: Glucose oxyhydrase, Beta-D-glucose:oxygen 1-oxido-reductase, gox