Horseradish Peroxidase / HRP Sheep Polyclonal Antibody

CAT#: AP09103AF-N

Horseradish Peroxidase / HRP sheep polyclonal antibody, Azide Free


USD 350.00

3 Days*

Size
    • 2 ml

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Specifications

Product Data
Applications ELISA, IP, WB
Recommended Dilution ELISA: 1/10000-1/40000. 
Dot Blot. 
Western Blot: 
1/1000-1/4000.
Immunoprecipitation.
Host Sheep
Isotype IgG
Clonality Polyclonal
Immunogen Native Peroxidase [Horseradish]
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase [Horseradish]. 
Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
Formulation 0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4
State: Azide Free
State: Lyophilized antiserum prepared from monospecific antiserum by a Delipidation and Defibrination.
Stabilizer: None
Preservative: None
Reconstitution Method Restore with 2.0 ml of deionized water (or equivalent).
Concentration 85.0 mg/ml (by Refractometry)
Background Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems.
HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.