Luciferase (Photinus pyralis) Goat Polyclonal Antibody

CAT#: AP09104PU-S

Luciferase (Photinus pyralis) goat polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/200-1/1000.
  Immunofluorescence: 20 µg/ml.
  Western Blot: 1/1000-1/5000.
  Immunohistochemistry: 1/1,000-1/5,000.
  Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, immunocytochemistry, and most immunological methods requiring high titer and specificity.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Luciferase Photinus pyralis (Firefly)
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  State: Purified
  State: Liquid (sterile filtered) purified IgG fraction
  Stabilizer: None
  Preservative: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Ion Exchange Chromatography
• Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.