GST-Tag Goat Polyclonal Antibody

CAT#: AP09205HR-N

GST-Tag goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and most immunological methods requiring high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/20,000.
  Western Blot: 1/2,000-1/5,000.
  Immunohistochemistry: 1/500-1/2,000.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Full length GST isolated from Schistosoma japonicum.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
  Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer.
• Conjugation: HRP
• Background: GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
• Synonyms: Glutathione S-transferase Tag, GST26-Tag