KLH Rabbit Polyclonal Antibody

CAT#: AP09215BT-N

KLH rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western BLot: 1/5,000 - 1/25,000.
  ELISA: 1/250,000.
  Immunohistochemistry.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: KLH (Keyhole Limpet Hemocyanin)
• Specificity: This antibody reacts to Keyhole Limpet Hemocyanin (KLH).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA) and 0.01% (w/v) Sodium Azide
  Label: Biotin
  State: Lyophilized purified Ig
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
  For extended storage add glycerol to 50%
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Affinity chromatography
• Conjugation: Biotin
• Background: KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
• Synonyms: Keyhole Limpet Hemocyanine