VSV-g Epitope Tag (YTDIEMNRLGK) Rabbit Polyclonal Antibody

CAT#: AP09231BT-N

VSV-g Epitope Tag (YTDIEMNRLGK) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA: 1:3,000 - 1:12,000.
  Western blot: 1:500 - 1:2,000.
  Immunoflourescence: 1:200 - 1:1,000.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding amino acids near the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G)
• Specificity: This antibody is directed against VSV-G protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; 10 mg/ml BSA (IgG and Protease free); 0.01% (w/v) Sodium Azide
  Label: Biotin
  State: Lyophilized Ig fraction
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
• Reconstitution Method: Restore with 0.2 ml of deionized water (or equivalent).
• Concentration: 0.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Purified from monospecific antiserum by immunoaffinity purification followed by biotin conjugation, subsequent purification and exhaustive dialysis against the buffer
• Conjugation: Biotin
• Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. VSV-G, or vesicular stomatitis virus glycoprotein, is found within the pseudo lentiviral cloning vector pHCMV-VSV-G.