FITC F(ab)2 Fragment Goat Polyclonal Antibody

CAT#: AP09428HR-N

FITC F(ab)2 Fragment goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  This product is designed for Immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  ELISA: 1/10,000-1/50,000.
  Western Blot: 1/2,000-1/5,000.
  Immunohistochemistry: 1/500-1/2,000.
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fluorescein conjugated to Goat IgG
• Specificity: Prepared from monospecific antiserum by Immunoaffinity Chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  Detects Fluorescein F(ab')2.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum and Fluorescein conjugated IgG.
  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified IgG F(ab')2 fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease Free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase (Molecular Weight 40,000 daltons)
• Reconstitution Method: Restore with 1.0 ml deionized water (or equivalent)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Imjmunoaffinity Chromatography
• Conjugation: HRP
• Background: Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (Fluorescein Isothiocyanate), carboxylates and succinimidyl esters.
• Synonyms: Fluorescein Isothiocyanate