Digoxigenin Sheep Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA |
Recommended Dilution | ELISA: 0.625μg/ml; 10 ng/ml digoxigenin produces 91% inhibition in a competitive ELISA, employing digoxigenin polyclonal antibody. |
Host | Sheep |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Digoxigenin-BTG |
Specificity | This antibody reacts to Digoxigenin. |
Formulation | 20mM Phosphate, 150mM Sodium Chloride, pH 7.2 containing 0.09% Sodium Azide as preservative State: Ig Fraction State: Liquid Ig fraction prepared by Caprylic Acid and Ammonium Sulphate precipitation procedures |
Concentration | 7.00mg/ml (U.V. abs @ 280nm) |
Background | Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. |
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