Kanamycin Sheep Polyclonal Antibody

CAT#: AP09735PU-L

Kanamycin sheep polyclonal antibody, Ig Fraction


USD 2,850.00

2 Weeks*

Size
    • 1 ml

Product Images

Specifications

Product Data
Applications ELISA
Recommended Dilution ELISA: 0.312 μg/ml.
Host Sheep
Isotype IgG
Clonality Polyclonal
Immunogen Kanamycin-BTG
Specificity This antibody recognizes Kanamycin.
Formulation 20mM Phosphate, 150mM Sodium Chloride
State: Ig Fraction
State: Liquid Ig fraction prepared by Caprylic Acid and Ammonium Sulphate precipitation procedures
Preservative: 0.09% Sodium Azide
Concentration 10.77 mg/ml (by U.V. abs at 280nm)
Background Kanamycin (also known as kanamycin A) is an aminoglycoside bacteriocidal antibiotic, available in oral, intravenous, and intramuscular forms, and used to treat a wide variety of infections. Kanamycin is isolated from the bacterium Streptomyces kanamyceticus and its most commonly used form is kanamycin sulfate.
Kanamycin is used in molecular biology as a selective agent most commonly to isolate bacteria (e.g., E. coli) which have taken up genes (e.g., of plasmids) coupled to a gene coding for kanamycin resistance (primarily Neomycin phosphotransferase II [NPT II/Neo]). Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin (50-100 ug/ml) containing agar plates or are grown in media containing kanamycin (50-100 ug/ml). Only the bacteria that have successfully taken up the kanamycin resistance gene become resistant and will grow under these conditions. As a powder kanamycin is white to off-white and is soluble in water (50 mg/ml).
Mammalian cells and other eukaryotes are screened using G418, a similar aminoglycoside antibiotic, which KanMX confers resistance against.
At least one such gene, Atwbc19 is native to a plant species, of comparatively large size and its coded protein acts in a manner which decreases the possibility of horizontal gene transfer from the plant to bacteria; it may be incapable of giving resistance to kanamycin to bacteria even if gene transfer occurs.
Synonyms Kanamycin A, Kantrex
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.