MASPIN (SERPINB5) Rabbit Polyclonal Antibody

CAT#: AP09877BT-S

MASPIN (SERPINB5) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human Maspin by Direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Maspin.
  Sandwich ELISA: To detect Human Maspin by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotinylated polyclonal antibody, in conjunction with Affinity purified Anti-Human Maspin (AP09877PU-N or AP09877PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Maspin.
  Western Blot: To detect Human Maspin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Maspin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified recombinant Human Maspin.
• Specificity: This antibody recognizes Maspin/SERPINB5.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction.
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C.
  Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: serpin family B member 5
• Database Link:
  Entrez Gene 5268 Human
• Background: MASPIN is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
• Synonyms: Serpin B5, PI5, PI-5
• Note: Centrifuge vial prior to opening.